Yuegang Zuo
University of Massachusetts Dartmouth, USA
Title: Determination of Glimepiride in Pharmaceutical Formulations Using HILIC
Biography
Biography: Yuegang Zuo
Abstract
Glimepiride is one of the most widely prescribed antidiabetic drugs and contains both hydrophobic and hydrophilic functional groups in its molecules, and thus could be analyzed by either reversed-phase high performance liquid chromatography (HPLC) or hydrophilic interaction liquid chromatography (HILIC) [Zuo Y (2014) (Eds.), High-Performance Liquid Chromatography (HPLC): Principles, Procedures and Practices. Nova Science Publishers, Inc., New York, USA; Zuo et al., Saudi Pharmaceutical J., 2017]. In the literature, however, only reversed-phase HPLC has been reported. In this study, a simple, rapid and accurate hydrophilic interaction liquid chromatographic method was developed for the determination of glimepiride in pharmaceutical formulations. The analytical method comprised a fast ultrasound-assisted extraction with acetonitrile as a solvent followed by HILIC separation and quantification. The effects of various HILIC parameters on the separation and determination will be discussed in details at the presentation. The developed method has been successfully applied to determine the glimepiride contents in pharmaceutical formulations and human fluids.