Mohamed Aly Zewail
National Research Centre, Egypt
Title: Synthesis Of C-Terminal Octapeptide B23-30 Of B-Chain Human Insulin By Classical Peptide Method To Be Used In Semisynthesis Of Human Insulin Iodinated At Tyrosine B16
Biography
Biography: Mohamed Aly Zewail
Abstract
The present work describes the preparation of protected octapeptide derivative (23-30) B-cain human insulin and coupling this peptide to B-chain desoctapeptide iodinated at tyrosine B16. B-chain will recombine with natural A-chain to give human insulin iodinated at tyrosine B16. Our approach of synthesis consisted of using stepwise coupling fragment condensation. All coupling was successfully with a high yield and easy to purify. For the side chain protection, we used (But) for OH-group of threonine and tyrosine, and (But) ester for C-terminal threonine. In most cases, Carbobenzoxy group was used for protection of N-terminal amino acids (Lys,Thr,Gly). Methylsulphonylethyloxycarbonyl (MSC) group was used for protection of NS-lysine.