Scientific Program

Conference Series Ltd invites all the participants across the globe to attend 2nd International Conference on Pharmaceutical Chemistry Barcelona, Spain.

Day 2 :

Pharmaceutical Chemistry 2017 International Conference Keynote Speaker Satinder Ahuja photo
Biography:

Dr. Satinder Ahuja successfully managed development of many new drugs at Novartis Corporation for over 25 years in a number of leadership positions that led to earnings of billions of dollars. His expertise is recognized worldwide in chromatography and separations, especially in the area of ultratrace analyses, chiral separations, and characterization of contaminants by various techniques. As President of Ahuja Consulting, he has advised major pharmaceutical companies in the US and abroad on new drug development and on green chemistry solutions to environmental issues. He has given plenary lectures worldwide, won awards, and published numerous papers and over twenty books.

Abstract:

New drug development may take as many as nine years and requires input from medicinal chemistry, analytical chemistry, chemical development, pharmaceutics, pharmacology, toxicology, manufacturing, marketing, and regulatory departments. The inputs from Analytical R&D are important for all of these operations because they can make the difference between slow and fast development. Drug discovery is generally initiated with the synthesis of a new chemical entity (NCE) based on combinatorial chemistry, or drugs are based on recombinant products. The molecular structure, including chirality, has to be confirmed. It is necessary to demonstrate the absence of any undesirable impurities, including enantiomers that may exhibit unusual pharmacologic or toxicologic activities (1–6). Finally, it is necessary to select an optimum dosage form, based on therapeutic and marketing needs. The selected dosage form has to meet GMP/GLP requirements. Discussion will focus on how various advances in chromatography and spectroscopy can help new drugs development and provide green chemistry-based solutions to a variety of problems encountered in the pharmaceutical industry.

Pharmaceutical Chemistry 2017 International Conference Keynote Speaker Fabio Marinelli photo
Biography:

Fabio Marinelli obtained his degree in Chemistry at the University  of Rome in 1980. In 1983 he became Organic Chemistry Researcher and, since 2001, he has been Associate Professor of Organic Chemistry at the University of L’Aquila. His research topics include the application of transition metals as catalysts in the synthesis of heterocycles such as indoles, quinolines, lactones and other. He has published more than 115 papers in reputed journals and is member of International Society of Heterocyclic Chemistry. 

Abstract:

Indole derivatives are one of the most extensively studied class of heterocyclic compounds. The indole nucleus is a fundamental constituent of many natural and synthetic products with biological activity. Moreover, fused indole derivatives display a number of interesting pharmacological properties. Cyclization of 2- alkynylanilines catalyzed by transition metals  represents a powerful tool for the  build-up of the indole nucleus, and affords mainly 2-substituded indoles, although sequential functionalizations of nucleophilic 3-position have been reported. An useful development of this methodology is represented by Pd-catalyzed reaction of 2-alkynyltrifluoroacetanilides with a variety of organic electrophiles such as aryl, heteroaryl, alkyl and alkynyl halides, vinyl triflates, arenediazonium tetrafluoroborates, boronic acids, a-iodoenones and  allyl esters. This approach is based on the activation of the triple bond towards intramolecular nucleophilic attack by –NHCOCF3 by means of coordination to an organopalladium(II) intermediate;  sequential reductive elimination results in the formation of 2,3-disubstituted indoles bearing the organic moieties linked to the -3 position). Furthermore, this methodology allows a straightforward assembly of polycyclic indole derivatives such as indoloquinazolines, indoloquinazolinones, and indoloquinolines. 

Keynote Forum

Markus R. Heinrich

Friedrich-Alexander-Universität Erlangen-Nürnberg, Germany

Keynote: New synthetic methods for medicinal chemistry
Pharmaceutical Chemistry 2017 International Conference Keynote Speaker Markus R. Heinrich photo
Biography:

M. Heinrich has completed his PhD at Ludwig-Maxilians-Universität München (Germany) in 2003. After postdoctoral research at Ecole Polytechnique (Palaiseau/France) and habilitation at Technische Universität München (2009) he was appointed as a professor for pharmaceutical chemistry at the Friedrich-Alexander-Universität Erlangen-Nürnberg (Germany). He has published more than 75 papers in reputed journals and he currently serves as a speaker of the Emil Fischer Graduate school at the FAU.

Abstract:

Our research group is interested in developing new radical reactions with a strong focus on a later application in the field of medicinal chemistry. Key reaction types are alkene and arene functionalizations. While important contributions could be made in the area of the Meerwein arylations (carboamination, carbooxygenation, carbofluorination), our efforts in radical aryl-aryl coupling are mainly dedictated towards the improvement of reactivity and selectivity as well as the late stage functionalization of peptides. The recent discovery of phenylazocarboxylates as versatile bifunctional reagents not only offers attractive synthetic pathways regarding the preparation of 18-fluorine-labeled radiopharmaceuticals, it also provides multiple options for combinatorial synthesis. Moreover, we have been able to show that important building blocks for pharmaceuticals can be prepared in a highly sustainable way using nitrogen oxides. So far, our newly developed reactions have been applied for the synthesis of antimalarials, numerous GPCR ligands, enzyme inhibitors as well as diverse radiolabeled compounds for PET imaging.     

Pharmaceutical Chemistry 2017 International Conference Keynote Speaker Larisa Klapshina photo
Biography:

Larisa Klapshina received her Ph. D in 1992 from the Razuvaev Institute of Organometallic Chemistry of Russian Academy of Sciences, IOMC RAS, (Nizhny Novgorod, Russia). Currently she is senior researcher at  IOMC RAS and at the Laboratory of Optical Theranostics in Nizhny Novgorod State University). She  and her group work in the area of  organic and organometallic synthesis and the functional materials for biophotonics and biomedicine. She is author of about 100 artcle

Abstract:

  The various techniques available for cancer diagnosis and therapy are traditionally considered as separate approaches in medical care.  But  nowadays development of the multifunctional agents which combine modalities for cancer diagnosis, treatment and real-time monitoring of treatment progress is real imperative for specifically personalized medicine.  Here we report series of novel tetracyanotetra(aryl) porphyrasine dyes which are found to be red-emitting fluorescent ‘molecular rotor’ i.e. the fluorescence lifetime and the quantum yield of these macrocycles  strongly increase as a function of environment viscosity. On the other hand, they works as an efficient PDT sensitizer, i.e. it induces apoptosis and necrosis in cells  upon irradiation with red light through formation of singlet oxygen. We demonstrated that PDT in vitro and in vivo using cyano-aryl porphyrasine macrocycles  is accompanied by a significant viscosity increase by monitoring the fluorescence lifetime of the rotor. We suggest that this increase could be used as a completely new type of diagnostic and dosimetry tool during a PDT treatment providing feedback information about individual therapy status. In addition, the results of in vivo experiments showed that PDT sensitizers prepared on the cyano-aryl porphyrazine pigment platform bound to gadolinium cation demonstrate the potential to become an extraordinarily effective multimodal agent for theranostics, representing a new approach to PDT based on real-time monitoring of the therapy in combination with precise MRI /fluorescence diagnostics of tumours.

  • Renowned Speakers

Chair

Speaker Slots are Available for Day 2

Speaker
Biography:

Matthew D. Lloyd graduated with a DPhil from Oxford on clavulanic acid biosynthesis. Following post-doctoral research at Brown University, U.S.A. and Oxford, he took up a lectureship at the University of Bath in 2002. He is currently Senior Lecturer (Associate Professor) in Pharmacy & Pharmacology. Research interests include prostate cancer, chemical biology, lipid metabolism, enzymes and inhibitors as drugs. He has published >70 research papers and is an editorial board member of The Journal Of Enzyme Inhibition and Medicinal Chemistry and The World Journal of Biological Chemistry. He is also a 4th Black Belt (International Instructor) in the Ch’ang-Hon Taekwon-Do.

Abstract:

 α-Methylacyl-CoA racemase (AMACR; P504S) catalyses a key step in the degradation of branched-chain fatty acids and is important for the pharmacological activation of Ibuprofen and related drugs. Over-expression of AMACR correlates with tumorigenesis of many cancer types in particular, prostate cancer.  Therefore, inhibition of AMACR is a promising chemotherapeutic  strategy. Development of AMACR as a drug target has been hampered by the lack of a convenient biochemical assay for enzymatic activity, and therefore few inhibitors have been identified to date. We have developed a new, continuous colorimetric assay based on the elimination of 2,4-dinitrophenolate from a novel acyl-CoA substrate. Our fully developed enzyme assay can be performed in a high-throughput screening format using a microtitre plate. Our assay has been used to determine the kinetic parameters for the substrate, determine IC50 and Ki values for known inhibitors, reversibility of inhibition, and characterise irreversible inhibitors. IC50 values for ~30 known substrates and inhibitors were determined to reveal the first structure-activity relationship study against AMACR in which potency was related to the lipophilicity of the acyl-CoA side-chain. The most potent inhibitor was N-dodecyl-N-methylcarbamoyl-CoA (IC50 vs. AMACR = 400 pM). ‘High-throughput screening’ and IC50 determination of drug-like molecule libraries identified several new classes of inhibitors of AMACR. Our colorimetric assay now allows for screening and rational drug design approaches and full characterization of AMACR inhibitors as new agents against prostate cancer.   

Speaker
Biography:

Sergey Lednev has completed his PhD at the age of 27 years from P.G. Demidov Yaroslavl State University. He is an assistance lecturer at the Department of General and Physical Chemistry. He has published 2 papers in reputed journals.

Abstract:

Polar solvents are widely used in the pharmaceutical industry and in various fields of applied chemistry. The ability to dissolve many organic substances and the wide temperature limits of the liquid state make polar solvents essential components of reaction mixtures in industrial and biotechnological processes as well as the components of drugs. On the other hand, polar solvents determine the medium in which the chemical process proceeds, and often have a significant effect on its kinetics. Therefore, an important practical task is to take into account the influence of the medium polarity on the kinetics of chemical reactions.

Thus, the medium polarity can have a significant effect on the reactivity of unsaturated compounds during the oxidation by molecular oxygen. The study of this process is important both for chemical technology and for understanding the chemistry and biology of oxidative stress. The effects of nonspecific and specific solvation may have a significant effect on the mechanism of oxidation of unsaturated compounds in the medium of polar solvents. The addition of an oxidation inhibitor to the reaction mixture complicates these effects. The report discusses the results of a systematic study of specific and nonspecific solvation effect on elementary stages of the inhibited oxidation of unsaturated compounds (methyllinoleate, styrene, methyl methacrylate, butyl acrylate). Antioxidants are phenols (PhOH), aromatic amines (AmH), stable nitroxide radicals (>NO), and corresponding hydroxylamines (>NOH). The results were obtained using a modern analytical basis: high-sensitivity microvolume, FT-IR, and NMR spectroscopy.
The research is supported by RSF grant No. 14-23-00018.

Speaker
Biography:

Sangeeta Jagtap received her M.Sc.in Organic Chemistry and is a recipient of Gold Medal from Bombay University (1992), India. She completed her Ph.D. from National Chemical Laboratory, Pune, India, under the guidance of Dr. R. M. Deshpande. She worked as a visiting scholar at Stanford University, California, USA, under the guidance of Prof. Barry Trost. She has also completed a ‘Post Graduate Diploma in Industrial Program in Pharmaceutical chemistry and Production’ and has cleared many competitive examinations like NET, SET, GATE and the tests organized by BARC. Presently she is working as Associate Professor at Baburaoji Gholap College, Sangvi, Pune, India. Her research interests are methodology, organic synthesis and catalysis. She has publications in reputed journals like Org. Lett., Tet. Lett., Cat. Today etc. She has authored a book named ‘Pharmaceutical, Medicinal and Natural Product Chemistry’.

Abstract:

Curcumin, mainly isolated from turmeric, for long is known for its anti-inflammatory and antioxidant activity. It is also known to be effective against neoplasms, Alzheimer's disease, Hodgkin's disease. There are large numbers of references available in literature about its biological activity and mode of action etc. Contrary to the popular belief that turmeric to be used only in household or as a medicine, we found an entirely different dimension to the whole idea of its activity. Curcumin is found to be active as catalyst. When complexed with palladium, it acts as a catalyst for coupling reactions. It catalyzes efficiently the Heck and Suzuki reactions almost upto 90-95%. The respective products have been isolated in good yield. Thus Curcumin is found to act as an efficient ligand in catalysis study. 

Speaker
Biography:

Shaaban K. Mohamed has completed his PhD at the age of 32 years from Minia University and postdoctoral studies from Didsburg University School of Chemistry, Germany and Manchester Metropolitan University, UK.. He is member of RSC and received  the Knowlege Exchange award 2013, MMU, UK .  He has published more than 260 papers in reputed journals and has been serving as an editorial board member of repute  journals such as International Journal of Chemistry and Pharmaceutical Research, Greener Journal of Pharmacy and Pharmacology, and Journal of Pharmaceutical and Applied Chemistry. 

Abstract:

The emergence of multidrug resistant (MDR) bacteria has necessitated the development of novel groups of antibiotics that effectively block or subvert bacterial growth [1,2]. It has been reported that different efforts and diverse investments have been made to develop novel strategies for improving the concept of antibiotic delivery that could enhance the limited activity of those vital antibiotics against such types of bacteria [3,4]. In the present study, amoxicillin trihydrate and Neomycin sulphate were used for the first time as both reducing and capping agents in synthesis of silver nanoparticles (AgNPs). The synthesized AgNPs were evaluated for their antibacterial and synergistic activity with antibiotics against selected human pathogenic bacteria.

Speaker
Biography:

Franz-Josef Meyer-Almes has completed his PhD at the age of 28 years from University of Goettingen. He has 10 years experiences in biotech and pharma companies. He is Professor for Physical Biochemistry and has published more than 40 papers in reputed journals and holds more than 10 patents and patent applications.

Abstract:

The importance of binding kinetics in terms of residence time and on-rate in drug discovery has been broadly accepted in the past few years. Furthermore, evidence has accumulated that the optimal binding mechanism of a drug to its target molecule is related to physiological efficacy as well as selectivity and thus drug safety. Homogeneous fluorescence-based binding assays have been shown to enable high throughput kinetics requiring only small amounts of protein and can be developed to elucidate even complex mechanisms of molecular recognition. A generalized approach is proposed that combines high quality kinetic and equilibrium data in an Integrated Global Fit analysis yielding the most probable binding mechanism.

Speaker
Biography:

El-Sawy has completed  her PhD at the age of 28 years from Al-Azher University and postdoctoral studies from National Research Centre, Cairo Egypt. She has published more than 34 papers in reputed journals. Current position: Professor Doctor of Organic Chemistry at Chemistry of Natural Compounds Department, National Research Centre (NRC). 

Abstract:

Resistance to conventional chemotherapy, leads to the need for development of novel safe and effective cancer therapies with new mechanism of action. Anti-angiogenic drugs are major example of such newly developed targeted therapeutics .In cancer drug development arena, coumarin-type compounds have been reported to bosses marked cytotoxic activities, in addition act as novel angiogenesis inhibitors. In this respect,a new series of coumarin derivatives was synthesized starting from 2-oxo-2H-coumarin-6-sulfonyl chloride (1), 6-nitro-2-oxo-2H-coumarin-3-sulfonyl chloride (10) and 6-amino coumarin-2-one (19). Reaction of 1 or 10 with 2-cyanoacetohydrazide, 2`-acetyl-2-cyanoacetohydrazide or 3-amino-5-pyrazolone afforded pyrazoline derivatives. While reaction of1 or 10 with malononitrile followed by reaction with hyadrazine hydrate, urea, thiourea or guanidine led to the formation of pyrazole and pyrimidine derivatives. On the other hand, compound 19 on reaction with VilsmeierHaack reagent yielded the corresponding aldehyde20. Compound 20 under reaction with chlorosulfonylisocyanate afforded N-chlorosulfonyl amid which cyclized to give pyranobenzothiazine derivative 25. The tested compounds 4, 5, 8, 12, 13 and 14 were non-cytotoxic against hepatocellular carcinoma cells (HepG2) using MMT. These non-cytotoxic compounds were evaluated as anti-angiogenicagent. Collectively, our results indicate that, coumarin molecules 4, 5, 8, 13 and 14 can be utilized as lead compounds to develop potential non-toxic angiogenesis inhibitors and small molecular ligands to target (HepG2), which was in concomitant with molecular docking results. 1-Acetyl-5-amino-4-(2-oxo-2H-chromene-6-sulfonyl)-1,2-dihydro-pyrazol-3-one (4) considered a promising anti-angiogenic agent, where it exhibited MMP-dependent anti-migratory activity and down regulated CD105.

Speaker
Biography:

Milan Mladenović has completed his PhD at the age of 27 years from University of Kragujevac, Republic of Serbia and postdoctoral studies the age of 27 from Sapienza Universiry of Rome. He is an Assistant Professor of Chemistry of Natural Products and Biochemistry at Faculty of Science, University of Kragujevac, and a Head of Department of Biochemistry. He has published more than 25 papers in reputed journals in the field of Biochemitry and Medicinal Chemistry. In the year of 2013 he became a director of Kragujevac Center for Computational Biochemistry, a specialized laboratory for molecular modeling and drug design.

Abstract:

Monoamine oxidase B (MAO B) catalyzes the oxidative deamination of aryalkylamines neurotransmitters with concomitant reduction of oxygen to hydrogen peroxide. Consequently, the enzyme’s malfunction induces oxidative damage to mitochondrial DNA and mediates development of Parkinson’s disease. Aiming to develope the novel reversible MAO B inhibitors, novel 128 compounds were rationaly designed after the modification of higly active co-crystallized coumarin inhibitor (Protein Data Bank ID: 2V61), utilizing the structure-based (SB) 3-D QSAR/SB and ligand-based (LB) alignment assesment/in vitro evaluation protocol. Coumarin 2V61 was improved by rules defined by 3-D QSAR models, generated from the available co-crystallized inhibitor-MAO B complexes, utilizing the 3-D QSAutogrid/R procedure: (1) an isobutyramide, as hydrogen-bond donnor towards MAO B Glu206, was introduced to the coumarin C3 position; (2) the C4 carbon was saturated with mixed hydrogen-bond acceptor/hydrophobic functions to interfere with recongition cage residues Tyr398 and Tyr435; (3) the C3=C4 double bond was improved with 1-methyl-6-oxopiperidine-2-carboxamide or 2-oxo-1,2,3,4-tetrahydropyrimidine-4-carboxamide rings and their methylated forms; (4) the C5 and C6 carbons were substituted by hydroxyl/methoxy functions to interact with Cys172; (5) the m-chlorobenzyloxyl moiety at C7, upgraded with o-, m-, or p-methyl groups, was retained to force the Ile199 in open conformation and to activate Phe103. Designed structures were SB LB aligned against MAO B, and six compounds were experimentally validated through synthesis and biological evaluation in vitro. Of them, two compounds, D123 (IC50 = 0.83 nM, K=0.25 nM) and D124 (IC50 = 0.97 nM, K=0.29 nM) were distinguished as potential lead candidates as anti-Parkinson’s drugs.

Speaker
Biography:

Zilhadia has completed his PhD from Faculty of Pharmacy Universitas Indonesia. She is a lecturer in Pharmaceutical Chemistry and the director of  Pharmacy Medicine Laboratory Syarif Hidayatullah State Islamic University since 2012 until now. She had joined the Syarif Hidayatullah State Islamic University since 2006. She has published several papers in reputed journals and becomes presenter in several international conferences.    

Abstract:

Vitamin C gummy is one of vitamin favoured by Indonesia Children. Gelatin is an important component for the manufacture of vitamin C gummy that serves as a gelling agent. Gelatin can be obtained by hydrolysis of collagen derived from skin, connective tissue and bones of animals from both bovine and porcine. Gelatine from porcine is forbidden for Moslems and Jews. The aim of this study was to differentiate between bovine and porcine gelatine in vitamin C gummy by High Performance Liquid Chromatography (HPLC) combined with PCA. Vitamin C gummy was hydrolyzed by 6 n-hydrochloric acid, then derivatized using 6-amino quinolyl-n-hydroxysucsinimidil carbamat (AQC) and analyzed by reversed-phase HPLC. The HPLC spectra were analyzed using a chemometric method, principal component analysis (PCA), to classify both of gelatin. The results from PCA, which were subsequently represented by the Cooman’s plot showed a clear distinction between gelatin samples of bovine and porcine origins.  This qualitative approach could determine the source of gelatin in food and pharmaceutical industries, especially in Vitamin C gummies.

Speaker
Biography:

Prakash Kinthada is a Professor in Chemistry at Sri Vidyanikethan Engineering college, JNTU University in Ananthapur, A. Rangam Peta, Tirupathi, India.

Abstract:

Cancer is a dreadful disease and any practical solution in combating this disease is of paramount importance to public health. Cancer patients have burdened by drug induced toxic side effects, and no turned to seek help from the complementary and alternative medicine hoping for a better cure. Research on Platinum based drugs and Non Platinum based drugs is a Multi-Million Dollar Industry in USA and there is every need to produce safe drugs for the cure of this monstrous disease. Flavonoids have a long history of use in traditional medicines in many cultures. The phytochemical, curcumin is one of the major dietary flavonoid, belonging to a group of flavonol, Curcumin is a natural polyphenol. It is highly potential molecule capable of preventing and treating various cancers.  Various dietary chemo preventive agents, turmeric powder or its extract are broadly used as therapeutic preparations in Indian System of medicine. We provide a summarized synthesis and structural determination of Curcumin Oxime, Curcumin Thiosemicarbazone derivative of Gold (III) complex. The use of these analogs for prevention of cancer tumor progression and treatments of human malignancies. A pharmacologic agent for treating and/or preventing cancer, among other diseases and conditions, and particularly breast, prostate, and pancreatic cancer, in humans and animals. The novel pharmacologic agent is an isoflavonoid or isoflavonoid mimetic covalently attached to a cytotoxic pharmacophore that, preferably has the ability to conjugate with a metal salt to form a more potent metal complex, particularly a Au (III) complex and other complexes of Platinum, Palladium, Ruthenium, Copper etc. My talk would mainly encompass different Transition Metal Complexes/Organometallic Compounds   that are presently used as drugs, especially Anticancer and Anti-HIV drugs, apart from Anti-inflammatory, Antimicrobial, Antibacterial and diseases like Arthritis and Parkinson’s Disease etc. The talk would mainly focus on the use of Medicinal Chemistry and it’s application to Drug Design and Development in Pharmaceutical Industry ,  especially    Transition Metal Complexes and Organometallic Compounds viz. Gold, Platinum, Palladium And Ruthenium apart from Copper, Cobalt, Iron,  Nickel, Zinc, Cadmium etc. The main emphasis of my talk would be on Different class of Ligands, their Schiff’s Bases and Transition Metal Complexes especially Au, Pt, Pd and Ru, with the main aim of designing, developing very novel small molecules, as possible and extremely potential candidates as Anti-cancer and Anti-HIV drugs. The talk would provide an overview of current programs being undertaken in our laboratories, especially focused on the development of potent ligands capable of recognizing Binding sites and diverse strategies employed by my group for elucidation of Anti-Cancer and Anti-HIV drug Leads to Circumvent the problem caused by Cis-Platin. We have synthesized and characterized several phytochemicals from Traditional Medicinal Plants and isolated some phytochemicals and  made the corresponding Oximes, Thiosemicarbazones and Substituted thiosemicarbazones as ligands and synthesized, characterized, structurally elucidated their Transition Metal Complexes especially with Gold, Platinum, Palladium, Ruthenium, Copper etc. and Studied their Anticancer Activity, Nuclease activity etc. and tested their potential as Anticancer Drugs. The main aim of our extensive/preclinical Pharmaceutical development program is to investigate the use of these extremely novel small molecules-metal complexes/compounds of phytochemicals, flavanoids etc., which have very interesting structural features and properties and hence are excellent candidates as Anti-Cancer and Anti-HIV drugs .The main aim of our research is Design ,Development and Synthesis of Transition Metal Complexes/ Organometallic Compounds that would certainly help to bring this force of nature from BENCH to BEDSIDE and enhance Cancer Killing with less toxic effects and would certainly lead to initiation of clinical trials.  

Speaker
Biography:

Shaima El-Mowafi has completed her PhD from the Pennsylvania State University in 2014. She is currently a postdoctoral researcher at the National Research Centre in Egypt.

Abstract:

One of the greatest threats to human health in the twenty-first century is the development of antibiotic resistance. Gram-negative pathogens are remarkably successful in evading antibiotic action, due to their ability to maintain the integrity of their cell envelope. Recently, a cyclic octapeptide, named SI24, was identified from a genetic screen as an inhibitor of the σE cell envelope-sensing pathway, which is required for the virulence and viability in several Gram-negative pathogens. SI24 was found to be toxic to E.coli bacterial cells when expressed in vivo, but was not, however, an effective inhibitor when added exogenously, probably because it cannot cross the cell envelope (1). On the other hand, positively charged antimicrobial peptides have been considered as potential therapeutic sources of future generations of antibiotics for treating resistant pathogenic microbes because of their broad-spectrum activities. These candidates have been developed as cell-penetrating peptides (CPPs) and have become one of the emerging vehicles for delivery of cargo drugs. We thus hypothesize that conjugation of the cyclic peptide inhibitor SI24 with CPPs could enhance its permeability, and consequently its activity against bacterial cells, providing a paradigm for the development of antibiotics targeting a novel pathway in Gram-negative pathogens.

Speaker
Biography:

Tetsuo Narumi has completed his Ph.D at the age of 28 years from Kyoto University with Prof. Nobutaka Fujii. He spent a year in US as a JSPS postdoctoral fellow with Prof. Jeffrey W. Bode at the University of Pennsylvania. In 2009, he began his academic career in Japan, at Tokyo Medical and Dental University with Prof. Hirokazu Tamamura. In 2013, he began his independent career at Shizuoka University, in Japan, as an associate professor in the Bioorganic Chemistry. He has published more than 50 papers in reputed journals and serving as a leading researcher in the fields of peptidomimetic science.

Abstract:

N-Heterocyclic carbenes (NHCs) have become widely utilized as organocatalysts in the formation of challenging C-C bonds. In particular, conjugated Breslow intermediates, derived from the reaction of a,b-enal and NHCs, have emerged as a powerful tool for a variety of transformations. Since the concept of conjugated umpolung through the intermediacy of catalytically generated homoenolates and activated carboxylates, many investigations have revealed their utility to synthesize various heterocycles such as γ-butyrolactones, γ-lactams and cyclopentenes. However, there are few methods for tuning the catalytic reactivity of NHCs by either rational design of NHC catalysts or appropriate additives for cooperative catalysis. This is particularly true of imidazolylidene catalysts, only a few members of which are utilized effectively for NHC catalysis. In this study, structural and kinetic investigation of a series of imidazolyli-dene catalysts with various N-aryl groups for homoenolate-mediated g-butyrolactone formation. Our study revealed that the reaction rate and catalytic reactivity can be clearly increased by the combination of 2,6-diethylphenyl groups as N-aromatic rings and a small excess amount of DBU as base compared to the originally reported conditions. Details of the structural studies, mechanistic investigations including parallel KIE experiments, and applications to the design of novel imidazolylidene catalysts will be discussed.

Speaker
Biography:

Dr Tarek Aboul-Fadl received his Ph.D. in Pharmaceutical Medicinal Chemistry from Assiut University/Egypt (1994) under the channel system and joint supervision scheme between Assiut University and Josai University/Japan. He performed his postdoctoral training as a postdoctoral research fellow and Scientist at Institute of Pharmaceutical Chemistry, University of Vienna, Austria (1997- 1998), Institute of Pharmacy and Food Chemistry, University of Erlangen-Nürnberg, Germany (1999 and 2013) and Department of Medicinal Chemistry, University of Utah, USA (2001-2002 and 2004-2005). He joined Department of Medicinal Chemistry as an assistant Prof. in 1994, then promoted to associate Prof. in 1999 and to Professor  in 2004. He has published more than 75 papers in reputed journals and serving as an editorial board member of repute. He has also registered 4 patents.

Abstract:

Asthma is a major public health issue with high and increasing prevalence rates and a concomitant increase in morbidity and mortality. Asthma is estimated to affect 300 million people, with an expected increase to 400 million worldwide by 2025. Many factors may have contributed to the rise of the problem of bronchial asthma. Increasing air pollution, fast modernization, and widespread construction work are some of the reasons for asthma to thrive. The situation is complicated by poor access to medical services and high price of effective drugs. Asthma is a chronic inflammatory condition, triggered by environmental factors in genetically predisposed individuals, and is characterized by mast cell, T lymphocyte, and eosinophil infiltrates in the bronchial mucosa. Eosinophils are recruited to sites of specific inflammatory reactions, especially during allergic diseases and are correlated with asthma severity.  In spite of  their numerous adverse effects inhaled glucocortocoids have been established as the standard treatment for asthma.  Therefore, an urgent need exists for alternative treatments to overcome these undesirable side effects of steroid therapy and to provide another effective agent for the treatment of asthma. Lidocaine inhibits interleukin-5 (IL-5)-mediated survival and activation of human eosinophils, and it is able to replace inhaled glucocorticoids for the treatment of asthma; however, lidocaine has many undesired side effects mainly due to its sodium channel activity including anesthesia. Accordingly, the current work aims to modify lidocaine structure to obtain analogs with minimum sodium channel IL-5 inhibitory activity. The hypothesis supported by ligand-based pharmacophore modeling generated using different molecular modeling programs.

Lilia Clima

Centre of Advanced Research in Bionanoconjugates and Biopolymers, Romania

Title: Dynamic combinatorial approch as synthetic strategy for the formation of non-viral vectors for gene therapy.
Speaker
Biography:

Abstract:

The next level in Drug Discovery is the easy building and self-generation of multifunctional nanostructures from commercially available or “easy to prepare” units, which will further self-assemble in a complex, tunable and multifunctional materials, suitable for very specific targeted drug delivery. Many functional platforms have been rationally designed with the hope of mimicking the complicated DNA histone machinery. However, DNA and target cells are highly variable and rational design is limited to a relatively small number of components and a high number of synthetic steps. One possible solution to this problem is to employ a dynamic screening approaches. In presented work adaptive dynamic vectors based on polyethylene glycol, cationic moiety components and in some cases squalene derivative, which are reversibly connected to core centres are prepared and tested as vectors for DNA transfection. Depending on their tuneable composition, these modular vectors dynamically self-adapt to their DNA targets, allowing the rapid screening of most effective vectors, optimally matched to DNA 3D surrounding space. Our strategy allows easy and efficient identification of adaptive vectors with high DNA complexation ability, good transfection efficiency, and well tolerated by mammalian cells.
This work was supported by Horizon 2020 WIDESPREAD 2-2014: ERA Chairs Project no 667387 and a grant of the Romanian National Authority for Scientific Research and Innovation, CNCS/CCCDI – UEFISCDI, project number PN-III-P3-3.6-H2020- 2016-0011, within PNCDI III.

Speaker
Biography:

Abstract:

As per World Cancer Report, ~14 million new cases of cancer occurred globally resulting ~15% of deaths in 2012 and over 100 cancers affect human being. Towards our research goal in improving the quality of life for the people suffering from cancer, we are working on two approaches:
A] DNA integrity is critical for proper cellular function & proliferation and has thus played a key role as successful molecular target for many of the drugs used in cancer therapeutics for decades. Targeting non-canonical DNA secondary structures such as G4s is now considered as an attractive approach toward drug intervention in anti-cancer therapy. Accordingly, significant research is in progress targeting G4 DNAs with small molecules,
hoping to inhibit cancer growth and having lesser side effects.
B] Protein-protein interaction (PPI) is of high importance in the regulation of cellular processes and is consequently implicated in the development of various disease states including cancer. The interaction between protein molecules leads to the formation of larger protein complexes performing a specific function and the first PPI inhibitor has entered in
clinical development.

Speaker
Biography:

Volodymyr Tkach, 28 years old,  has completed his PhD at the age of 25 years from Chernivtsi National University, Ukraine and postdoctoral studies from Federal University of Mato Grosso do Sul, Brazil. He speaks Ukrainian, Russian, English, Portuguese and Spanish. He has published more than 80 papers in reputed journals and his scientific interests includes the development of new conducting polymers and composites for pharmaceutical compounds’and pesticides’ analysis, like also the electroanalytical investigation of cobalt-based materials and their composites with conducting polymers and carbon materials.

 

Abstract:

Uric acid is the final product of the genetically determinated process of purine metabolism in the human and vertebrate animal organism. Its lack leads to the hyperuricemia and pathological states like Wilson and Fanconi disease. On the other hand, its excess leads to the Lesch-Nyhan syndrom. It makes actual the task of the seek of the efficient mechod of its concentration determination in organism. The redox activity of the uric acid let us apply the electroanalytical methods to it, especially those using the chemically modified electrodes, as the use of the modifiers accelerates the process and makes it more efficient. The cobalt(II)-tin(IV)-hydroxide is an interesting and modern electrode modifier, recently obtained in cubic nanopearticles. It is seen as an interesting candidate to catalyse the photo, photoelectro and electrochemical systems (including the sensing systems). Its use for the detection of the substances, which are oxidized in the similar conditions let us investigate theoretically the determination  the possibility of the electrochemical detection of uric acid over an electrode, modified with this material in this work. The behavior of this system may be described by a tridimensial equation  set, whose analysis shows that the electroanalytical system is driven efficiently, and may be diffusion- or reaction-controlled. The material may be used solely or in a composite with conducting polymers (polyaniline, polypyrrole, polycarbazole etc) and carbon materials (especially carbon paste, MWCNT and graphene). The oscillatory behavior is possible and may only be caused by DEL-capacitances change. 

Speaker
Biography:

 Yusuf Al-Hiari has completed his PhD at the age of 31 years from Strathclyde University/UK and then appointed as assistant prof. At The faculty of Pharmacy, JU. He was promoted to full Prof. During 2012. He has published more than 65 papers in reputed journals  with impact factor, 6 patents and  has been serving as an editorial board member of repute journals.

Abstract:

More than 5 heterocyclic systems were revealed with potential hypolipidemic activity by our group such as indole, pyrrole, furan, benzofuran and pyridine. All derivatives prepared holds carboxamide attached to highly lipophilic substituents such as benzophenones and antharaquinones. This presentation aims to prepare new 5-substituted indole-1H-indole-2-carboxamide derivatives I as a model and to investigate their hypolipidemic activity in-vivo using Triton WR-1339-induced hyperlipidemic rats as animal model. The novel compounds involve 5-Fluoro-1H-indole-2-carboxamide derivatives of benzophenones1 (series A; 5, 6, 12-15), anthraquinones2 (series B; 17, 19), anilines (series C; 21, 25) and acetophenones (series D; 26-27). The new derivatives represented by compounds 5, 6, 17m and 19 have shown a significant reduction in triglyceride levels (69-90%) compared to hyperlipidemic rats. The analogous 5-Chloro , 5-Bromo and 5-methoxy-1H-indole-2-carboxamide derivatives did not change the pattern of activity with  all active derivatives. The elevated plasma triglyceride levels produced by Triton WR-1339 administration were significantly (p < 0.0001) suppressed in bezafibrate (79%), in compound (5) (90%), in compound (6) (83%),  in compound (17m) (69%) and (79%) in compound (19) after 12 h in comparison to hyperlipidemic control (HG). At the same time, high-density-lipoprotein cholesterol levels were significantly increased after 12 h of Triton administration (+ 86% p < 0.0001) in compound (5),  (+ 53% and +27% p<0.01) in compound (6), BF and (+ 16% and + 37% p<0.05) in compound (17m) and (19) respectively compared to hyperlipidemic control (HG).

 

Speaker
Biography:

Shirly Kumala has completed her PhD from Biomedic Faculty, University of Indonesia. Jakarta. She is the Dean of Pharmacy Faculty. University of Pancasila. Jakarta, Indonesia. She has published more than 25 papers in both international and national journals, and has been serving as a reviewer in Journal of Pharmacy. 

Abstract:

Microorganisms, in particular, endophytic microbes have been well documented as alternative source of raw materials for drug development.  Secondary metabolites produced by endophytic microbes have efficacious medicinal activity. The aims of this research focussed on isolation of the endophytic microbes from Bawang dayak leaf and evaluated their secondary metabolites. Isolation of endophytes were performed in PDA (Potato Dextrose Agar) using direct seed plant method. Endophytic fungi isolates with strongest antioxidant activity were fermented in PDY (Potato Dextrose Yeast) to produce large scale of the metabolites.  Supernatant were extracted with ethyl acetate solvent. Ethyl acetate extract fractionated by column chromatography {SiO2, (i). chloroform –methanol = 50:1~10:1; (ii). Choloroform-Methanol = 5:1) and obtained three fractions. Further, colorimetric using free radical scavenger method was perform to assess their antioxidant activity. The highest antioxidant activity were identification by Nuclear Magnetic Resonance (1H- & 13C-NMR), infrared and mass spectroscopies showed the antioxidant compoud as flavonoid. In conclusion, the pure compound of secondary metabolites isolated from Bawang dayak leaf is flavonoid that could be responsible for its potent antioxidant activity.

Speaker
Biography:

Abstract:

Pharmacological toolsÐ`chemical probes'Ðthat intervene in cell signaling cascades are important for complementing genetically-based experimental approaches. Probe development frequently begins with a high-throughput screen (HTS) of a chemical library. Herein, we describe the design, validation, and implementation of the first HTS-compatible strategy against any inositol phosphate kinase. Our target enzyme, PPIP5K, synthesizes `highenergy' inositol pyrophosphates (PP-InsPs), which regulate cell function at the interface between cellular energy metabolism and signal transduction. We optimized a timeresolved, fluorescence resonance energy transfer ADP-assay to record PPIP5K-catalyzed, ATP-driven phosphorylation of 5-InsP7 to 1,5-InsP8 in 384-well format (Z' = 0.82 ± 0.06). We screened a library of 4745 compounds, all anticipated to be membrane-permeant, which are knownÐor conjectured based on their structuresÐto target the nucleotide binding
site of protein kinases. At a screening concentration of 13 μM, fifteen compounds inhibited PPIP5K >50%. The potency of nine of these hits was confirmed by dose-response analyses. Three of these molecules were selected from different structural clusters for analysis of binding to PPIP5K, using isothermal calorimetry. Acceptable thermograms were obtained for two compounds, UNC10112646 (Kd = 7.30 ± 0.03 μM) and UNC10225498 (Kd = 1.37 ± 0.03 μM). These Kd values lie within the 1±10 μM range generally recognized as suitable for further probe development. In silico docking data rationalizes the difference in affinities. HPLC analysis confirmed that UNC10225498 and UNC10112646 directly inhibit PPIP5K-catalyzed phosphorylation of 5-InsP7 to 1,5-InsP8; kinetic experiments showed inhibition to be competitive with ATP. No other biological activity has previously been ascribed to either UNC10225498 or UNC10112646; moreover, at 10 μM, neither compound inhibits IP6K2, a structurally-unrelated PP-InsP kinase. Our screening strategy may be generally applicable to inhibitor discovery campaigns for other inositol phosphate
kinases.

Speaker
Biography:

Abstract:

Reaction of 4-aryl-3-cyano-5-ethoxycarbonyl-6-methylpyridine-2(1H)-thiones 3a,b with chloroacetonitrile gave the corresponding 3-aminothieno[2,3-b]pyridine-2-carbonitriles  5a,b. Condensation of  5a,b with triethyl orthoformate produced the methanimidate derivatives 7a,b. Treatment of 7a,b with hydrazine hydrate at room temperature resulted in the formation of ethyl 3-amino-9-aryl-3,4-dihydro-4-imino-7-methylpyrido[3’,2’:4,5]thieno[3,2-d]pyrimidine-8-carboxylates 8a,b. 3-Amino-4-(4-methoxyphenyl)-5-ethoxycarbonyl-6-methylthieno[2,3-b]pyri-dine-2-carboxamide (6) was prepared and reacted with triethyl orthoformate to give pyrimidine-4(3H)-one derivative 15. Chlorination of 15 with phosphorus oxychloride gave 4-chloropyrimidine 16, which in turn was reacted with hydrazine hydrate to produce ethyl 4-hydrazino-9-(4-methoxyphenyl)-7-methylpyrido[3’,2’:4,5]thieno [3,2-d]pyrimidine-8-carboxylate (18). Compounds 8a,b and 18 were used as precursors for synthesizing other new pyridothienopyrimidines as well as triazolopyridothieno-pyrimidines, and pyridothienopyrimidotriazinoindoles. Structural formulas of all newly synthesized compounds were confirmed by elemental and spectral (IR, N MR and mass) analyses.

Speaker
Biography:

He has completed his PhD at the age of 34 years from University Federal of Rio de Janeiro and postdoctoral studies from Univesity Federal Fluminense. He has published more than 20 papers in reputed journals.

Abstract:

In this work, we present a new GIAO-HDFT universal scaling factor (mPW1PW91/3- 21G//PM7(I)) and a comparative study in which is investigated its ability to predict NMR 13C chemical shifts (δ) with high cost-effectiveness ratio. A set of 22 small molecules providing 27 different 13C δ determined in the gas phase was used for all scaling factors protocols: B3PW91/ccpVDZ//B3PW91/cc-pVDZ (II), B3PW91/cc-pVTZ//B3PW91/cc-pVDZ (III), B3LYP/6- 311+(2d,p)//B3LYP/6-31G(d) (IV), mPW1PW91/6-31G(d)//PM7(V) , mPW1PW91/6- 31G(d)//mPW1PW91/6-31G(d) (VI). Despite the calculation approximations the δ calculated at the
GIAO-mPW1PW91/3-21G//PM7 using a simple relationship (δscal = 1.14 . δcalc – 4.7, where δcalc and δscal are the calculated and the linearly scaled values of the 13C δ, respectively) were able to yield MAD and RMS errors as small as those obtained with other GIAO-HDFT with bigger basis sets
(protocols (II) to (VI)). The robustness of the new protocol and its applicability to practical problems was evaluated by the calculation of the δ for two natural compounds with synthesis, biological and therapeutic interest: tryptanthrin (indolo[2,1-b]quinazoline-6,12-dione) and (-)- loliolide (7aR)-6-hydroxy-4,4,7a-trimethyl-6,7-dihydro-5H-1-benzofuran-2-one. For both compound, the 6 protocols presented good agreement with experimental data. Moreover, for the second compound, the new protocol performs even better than the 5 others. In conclusion, GIAOmPW1PW91/ 3-21G//PM7 linear regression obtained by using the experimental and the calculated data, is a very attractive tool as an alternative to more computationally demanding approaches, which are usually applied in order to achieve 13C NMR δ calculations.

Speaker
Biography:

Abstract:

This study was aimed at evaluation of phytochemical constituents and the effect of ethanol leaf extract of Piliostigma thonningii on the central and peripheral nervous systems in laboratory animals. Fresh leaves of Piliostigma thonningii were air-dried, pulverized extracted using soxhlet extraction technique with ethanol 148.24% w/w after being concentrated. The extract was screened for phytochemicals using standard methods. 20 g of the ethanol extract was subjected to column chromatographic (CC) analysis using ethyl acetate and n-butanol as mobile phase at different ratios and silica gel of 60-120 mesh as the stationary phase. Fractions obtained with similar retention factor (Rf) using thin layer chromatography (TLC) were combined, coded and subsequently screened for phytochemicals. Subsequent purification of fraction PTE3 was carried out using CC (ethylacetate and methanol were used as mobile phase at different ratios) and TLC until a sub-fraction PTE34 amongst other fractions gave a single spot on TLC and had a melting point of 102-103 oC. The phytochemical studies of the ethanol leaf extract of Piliostigma thonningii revealed the presence of some useful chemical compounds such as flavonoids, cardiac glycosides, tannins, saponins, and terpenoids. The pharmacological effects of Piliostigma thonningii was determined by examining the effects of the leaf extract on phenobarbitone sleeping time, analgesic and muscle relaxant activities using experimental animals. The analgesic effect of the leaf extract was evaluated with acetic acid induced writhing and thermally induced Nociception for pain. It was observed that the extract conferred 48.00 and 57.20% protection from writhes induced by acetic acid on mice when extract doses of 200 and 400 mg/Kg were administered. Similarly, there was a significant (p<0.5) dose dependent effect conferred on mice when pain was induced by heat. The extract also had a muscle relaxant effect as 20%, 60% and 80% were observed to slide down an inclined board in a dose dependent manner. The extract also significantly potentiated sleeping time of phenobarbitone dose dependently in rats of which the mean time duration of (72.0±04.64) min, (83.40±02.11) min, and (123.60±11.57) min were observed when rats were administered extract doses of 200, 400 and 600 mg/Kg b wt. Thus the ethanol leaf extract of Piliostigma thonningii was able to provide depressant effects which were shown in its ability to potentiate barbiturate sleeping, analgesia and muscle relaxant effect. 

Speaker
Biography:

Dr. Aramice Y. S. Malkhasian completed Ph.D. at  Concordia University 1985and did Research scientist at McGill with M. A. Whitehead  at 1995,  at 2003 and 2004 work with Prof Michael Sevillaand Prof. Ferman Chaves at Oakland university. Currently professor at King Abdulaziz University
 

Abstract:

Medicinal chemistry has in the past been dominated by learned insights from experienced organic chemists. However, with the advent of computer based methods, computer aided drug design has become prominent. We have compared here the ability of expert chemists to purely automated methods and found that the automated method produces a better potential candidate drug than the expert input. The example chosen is based on inhibitors to Abl-kinase and the successful anti-leukaemic drug imatinib. The proposed molecule is a simple modification of nilotinib and has a docking energy of 4.2 kJ/mol better than the best intuitive molecule

Speaker
Biography:

Abstract:

Tiacumicin B is an antibiotic endowed with the remarkable ability to interact with a new biological target giving it an inestimable potential in the context of the ever-growing and worrisome apparition of resistances of bacteria and mycobacteria to antibiotics. The development of an efficient synthesis of this complex molecule will allow accessing valuable analogues. We have achieved the total synthesis of the tiacumicin B aglycone featuring the DFT-guided strategy concept. Macrolactone thus obtained is ready for subsequent glycosylation step. Starting from known alcohol (±)-3, this 16 steps synthesis was performed in a 3.6 % overall yield, only 4 steps dealing with the installation or the removal of protective groups. Relying on DFT predictions, we dared to use an unprecedented [2,3]-Wittig rearrangement of the propargyl ether of tertiary allylic alcohol to synthesis the most densely functionalized fragment of the target. We also accessed the tetrasubstituted C12-C15 diene stereoselectively using an innovative strategy based on the sequence allene-alkyne Pd/Cu-catalyzed cross-coupling / selective hydrosulfuration / Pd-catalyzed Kumada-Corriu cross-coupling of an alkenylsulfide function. To end this synthesis the E configuration of the C4=C5 bond was controlled thanks to a selective cross-metathesis of vinylborate, and a Suzuki cross-coupling was used to install the missing C1-C3 fragment. The final macrolactonization step was found to be ring-size-selective as again predicted by DFT.

Speaker
Biography:

My name is Ntsoaki Anna ‘Nyane ,I am African female aged 25 years old, who studied BPharm Degree at University Of Lesotho. I enrolled in my studies at University of KwaZulu-Natal where I am currently doing my Research under the department of Pharmacology, School of Health Sciences. I am in my second year of Masters. I was awarded first prize (30000-travel voucher) in research symposium 2016 for winner in the Masters oral category held by College of Health sciences. I have also assisted the Honours students with their Laboratory Experiments. I am doing lecturing on third level and fourth level at University of KwaZulu-Natal in school of Pharmacy. I have two accepted manuscript in Journal of Pharmacology and the other is PLOS ONE.

Abstract:

Type 2 diabetes (T2D) is characterized by impaired insulin secretion and peripheral insulin resistance. Despite many classes of drugs available, T2D is still projected to increase by 55% in 2035. Citrus fruit-derived flavonoid, naringenin has been reported to have antidyslipidemic, anti-oxidant and more recently metformin-like antidiabetic effects. Metformin, the most commonly used drug for T2D management acts by activating adenosine monophosphate activated protein kinase (AMPK). Naringenin’s anti-diabetic effects could be mediated by AMPK activation. Although naringenin has been shown to have anti-diabetic properties, it is less lipophilic and has poor water solubility hence chalco-naringenin analogs with enhanced pharmacological activities were synthesized. A series of 11 compounds of 4-[(cyclopropylcarbonyl) amino] chalco-naringenin analouges were synthesized using Claisen-Schmidt and characterized by IR, 1H-NMR and 13 C-NMR. An intermediate compound, N-(3-acetylphenyl) cyclopropanecarboxamide synthesized was reacted with commercially available aldehydes to yield the final amnio-chalco-naringenin series. The synthesized compounds showed characteristic peaks on IR, 1H-NMR and 13 C-NMR and fit very well in the hydrophobic binding pockets of AMPK. They also presented good binding affinity to the enzyme as shown by computer simulation suggesting potential metformin-like antidiabetic effects.
Further in vitro and in vivo antidiabetic studies are suggested to elucidate the molecular mechanisms of these compounds.

Speaker
Biography:

Abstract:

Multicomponent reactions (MCRs) receive increasing attention because they address both diversity and complexity in organic synthesis. With these one-pot reactions diverse sets of relatively complex structures, especially heterocycles, can be generated from simple starting materials. In many MCRs (e.g. the Ugi reaction), isocyanides are important building blocks. Recently, isocyanides have found also application as versatile C1 building block in palladium catalysis. These reactions offer a vast potential for the synthesis of nitrogen containing fine chemicals. In this presentation, the development of novel atom- and step efficient Pd-catalyzed reactions involving isocyanide insertion will be presented. Further, in order to address stereoselectivity issues connected to certain MCRs, biocatalysis offers unique opportunities.  Recently, we have developed several methods based on the enzymatic desymmetrization of meso-pyrrolidines using a monoamine oxidase N (MAO-N) from Aspergillus niger optimized by directed evolution and its combination with highly diastereoselective Ugi-type three-component and Ugi-Smiles reactions.  In this presentation we highlight several aspects of this chemistry in the context of heterocycle synthesis with applications in green chemistry and pharmaceuticals.

Speaker
Biography:

Nurmeilis has completed her doctoral studies (Dr) at the age of 41 years from Universitas of Indonesia with majoring of pharmacy  and work as a lecturer in pharmacy department, Faculty of medicine and health sciences, Syarif Hidayatullah State Islamic University and now as a Head of program study of pharmacy.

Abstract:

Ethyl p-methoxycinnammate (1) was found as a major isolated compound from the rhizome of Kaempferia galanga. This compound has been reported to have various biological activities such as mosquito repellent and larvicidal, anti-tuberculosis, sedative, anticancer, analgesic and anti-inflammatory and hypo pigmentary. In this research, we have evaluated the sedative and hypnotic activity of 1 and its amide derivative, N-hydroxyethyl-p-cinnamamide (2). The method was carried out by evaluating significant sedative and hypnotic effect of  1 and 2 at the doses of 100, 200 and 400 mg/kg (by oral route), compared to reference substance diazepam in hole board and thiopental-induced sleeping time methods. In conclusion, compound 1 has 2 has a potential sedative and hypnotic activity, whereas compound 1 was significantly different to the negative control (p≤0,05)

Alvaro Escribano

Universidad Carlos III de Madrid, Spain

Title: Score-driven dynamic patent count panel data models
Speaker
Biography:

Abstract:

In this paper, we propose the use of Dynamic Conditional Score (DCS) count panel data models. We compare the statistical performance of the static model with different dynamic models: finite distributed lag, exponential feedback and different DCS models. For DCS, we consider random walk or quasiautoregressive
dynamics. We use panel data for a large cross section of United States firms for period 1979–2000, and the Poisson quasi-maximum likelihood estimator with fixed effects. The empirical results suggest that DCS has the best statistical performance.

Speaker
Biography:

Abstract:

 This study is aimed at evaluating the biochemical effects and antioxidants activity of extracts of Vernoia calvoana Hook. f (V.C) on STZ induced diabetic rats. Thirty-six (36) rats weighing (100-150g), were divided into 6 groups of 6 animals each. Groups 1 and 2 representing normal and diabetic controls (NC and DC) respectively, receiving placebo, while groups 3-6 represented diabetic treated, receiving 500 mg/kg b.w metformin, 400 mg/kg b.w crude, n-hexane and methanol fractions of V.C, respectively. Treament with drug and extracts of V.C showed a decrease in fasting blod glucose (FBG) in all experimental groups and was significant (p<0.05) on the 7th day of the experimental period, compared to diabetic control. Progressive increase in body weight was observed in all experimental groups compared to DC group. A significant (p<0.05) increase in glutathione peroxidase (GPX) and catalase (CAT) activities were recorded in all experimental treated animal compared to DC and NC. Malondialdehyde (MDA) concentration was observed to decrease significant (p<0.05) in all experimental groups compared to DC. Histopathologically, the changes in pancreatic integrity was consistent with that of biochemical findings. It may be concluded that, extracts of V.C possess potent ameliorative activity against STZ-induced diabetes, via a potential free radical mopping activity.

Speaker
Biography:

My name is Mohammed Alqarni. I am PhD student in pharmaceutical analysis (2nd year) under supervision of Dr. Mounir Maafi (Leicester School of Pharmacy).

Abstract:

far despite the regulatory status of the photostability testing of drugs as stipulated by the ICH in its Q1b section [1]. The lack of clarity in the ICH recommendations makes it necessary to develop concise and rational approaches to quantify the photodegradation of drugs [2-4]. In this study, we
present for the first time, a mathematical framework that describes the photokinetics performed under polychromatic light and its validation by experimental results for a number of APIs. Our approach demonstrates the importance of the wavelength dependence of the absorption coefficient, the radiant power and the photochemical quantum yields in the evaluation of
photodegradation. Furthermore, the mathematical formulation proves that the classical kinetic treatments based on thermal 0th-, 1st and 2nd order reactions should not be used for photodegradation investigations. Evidence of an important photostabilization of drug is provided and attributed to the
increase of the initial drug concentration. These findings explain the usually low photodegradation observed for relatively highly concentrated pharmaceutical formulations but also underlines the importance of such studies for in vivo (low concentrated) drugs photostability. Our work can help standardise and amend the ICH procedures and recommendations for photostability testing.

Speaker
Biography:

Abstract:

Several formulation strategies for modifying the skin permeability and for potentiating the activity of an active compound can be envisaged. Diclofenac diethylamine is a nonsteroidal anti-inflammatory drug widely prescribed to treat mild and moderate pain, inflammation and osteoarthritis by inhibiting the enzyme cyclooxygenase. It is already marketed in various forms (gel, emulgel, ophthalmic solution, immediate and prolonged release tablet, suppositories and intramuscular injection). Because of its low solubility in water and in the acidic environment of the stomach, Diclofenac has a low availability and short half-life of about two hours. A topical application for the treatment of rheumatic diseases is therefore suggested to increase bioavailability and decrease toxicity. The objective of this work was to develop a liposomal suspension encapsulating a non-steroidal anti-inflammatory which will constitute the dispersed phase of a gel for dermic use. Diclofenac preparations for dermal application are only marketed in the form gel and emulgel. This liposomal form has been proposed in order to increase the skin penetration or even the availability of Diclofenac thanks to the phospholipidic bilayer of the liposome having the same Structure so that the skin can cross the skin barrier (horny layer). The liposomes were prepared from soy lecithin, cholesterol by the reverse- phase evaporation method. Then analyzed by electron scanning microscope, high-performance liquid chromatography, laser particle size and Zeta-metry to determine their size, encapsulation rate and zeta potential. An optimum with a maximum amount of ethanol in relation to water combined with maximum levels of cholesterol and lecithin was obtained, which promotes the production of small-sized liposomes containing a relatively high surface charge (zeta potential) and having only a narrow polydispersity, thus meeting the stability criteria favoring transposition on an industrial scale.

Speaker
Biography:

Yin Zhou is fourth-year undergraduate Chemical Biology student at the University of British Columbia. She has been an analytical Chemistry research assistant in Chen Research Group since September 2015, where she started her own research project to size cyclodextrins. She is also an Organic Chemistry tutor in the same college, science and Math tutor for high school students. She mentored 14 high school students with their science projects. She has been a social Psychology research assistant, being involved in Gerontology since 2015.
 

Abstract:

Sizing α, β and γ Cyclodextrins by Capillary Electrophoresis and Indirect UV Detection: Cyclodextrins have ring structures made of glucose connected by 1,4-glycoside linkage and they differ in the number of glucose on the ring. The interior of cyclodextrin is considerably less hydrophilic than its exterior; therefore, they are useful for carrying hydrophobic molecules. Due to their hydrophilic exterior, they are able to penetrate body tissues, which makes them good candidates as drug carriers where they can release biologically active compounds under specific conditions. However, different sizes of cyclodextrins form different complex with the same molecule, so, it is important to know the size of each cyclodextrin. Indirect UV-capillary electrophoresis and Taylor dispersion analysis are used to size α, β and γ cyclodextrins. Because cyclodextrins have low UV absorbance, indirect UV is used in which the background electrolyte has significantly higher UV absorbance than the target molecules, therefore, resulting in negative peaks. Using TDA, diameters of α- and γ-CD are calculated to be 0.70nm and 0.86nm. The small standard deviation indicated the precise and reproducible measurement. 

Azrifitria

Islamic State University (UIN) Syarif Hidayatullah Jakarta, Indonesia

Title: Spermicidal and Antifertility Activity of Costus Spiralis Leaf Ethanolic Extract in Male Rats
Speaker
Biography:

Azrifitria has completed  her  PhD at the Indonesia  University School of Medicine. She is the head of pharmacy department at islamic state University Syarif Hidayatullah Jakarta, Indonesia. She has published several  papers in reputed journals and get  some course at pharmacy field at tokushima bunry japan. 

Abstract:

Costus spiralis belongs to costus genus which is one of the source of steroidal saponin that can potentially be an antifertility agent. This study investigated the effect of Costus spiralis  leaf 70% ethanolic extract on male reproductive system using rat model. The study was divided into four groups of five adult male rats Sprague-Dawley aged 12 weeks;  control group, and treatment group those received 12.5; 25 and 37.5 mg/kg body weight of  Costus spiralis extract. The 70% ethanolic extract of Costus spiralis was administered orally  once a day for 48 days. Different parameters were studied including sperm motility, sperm count,  tubulus seminiferous diameter, spermatocyte pachytene at stage VII-VIII, serum testosteron and  spermicydal activity. The result of the analysis showed that the extract dosages had a significant difference (p ≤ 0,05) on the decrease of sperm motility, tubulus seminiferous diameter, sperm count and number  of spermatocyte pachytene at stage VII-VIII compared to control without commensurate decline in serum testosterone levels. The minimum effective concentration  of Costus spiralis  leaf 70% ethanolic extract to totally immobilise sperm within 20 seconds was 20 mg/mL. The results of present experiment suggested that the Costus spiralis  leaf 70% ethanolic extract exerted a significant anti-spermatogenic effect in male rat.