Zilhadia
Syarif Hidayatullah Islamic State University, Indonesia
Title: Differentiation of Bovine and Porcine gelatin in vitamin C gummy by High Performance Liquid Chromatography using Principal Component Analysis
Biography
Biography: Zilhadia
Abstract
Vitamin C gummy is one of vitamin favoured by Indonesia Children. Gelatin is an important component for the manufacture of vitamin C gummy that serves as a gelling agent. Gelatin can be obtained by hydrolysis of collagen derived from skin, connective tissue and bones of animals from both bovine and porcine. Gelatine from porcine is forbidden for Moslems and Jews. The aim of this study was to differentiate between bovine and porcine gelatine in vitamin C gummy by High Performance Liquid Chromatography (HPLC) combined with PCA. Vitamin C gummy was hydrolyzed by 6 n-hydrochloric acid, then derivatized using 6-amino quinolyl-n-hydroxysucsinimidil carbamat (AQC) and analyzed by reversed-phase HPLC. The HPLC spectra were analyzed using a chemometric method, principal component analysis (PCA), to classify both of gelatin. The results from PCA, which were subsequently represented by the Cooman’s plot showed a clear distinction between gelatin samples of bovine and porcine origins. This qualitative approach could determine the source of gelatin in food and pharmaceutical industries, especially in Vitamin C gummies.