Shirly Kumala
Pancasila University Srengseng Sawah, Indonesia
Title: Isolation and Characterization Active Compound From Endophytic Fungi Onion Dayak {Eleutherineamericana (aubl.) Merr} as Antioxidant Activity
Biography
Biography: Shirly Kumala
Abstract
Microorganisms, in particular, endophytic microbes have been well documented as alternative source of raw materials for drug development. Secondary metabolites produced by endophytic microbes have efficacious medicinal activity. The aims of this research focussed on isolation of the endophytic microbes from Bawang dayak leaf and evaluated their secondary metabolites. Isolation of endophytes were performed in PDA (Potato Dextrose Agar) using direct seed plant method. Endophytic fungi isolates with strongest antioxidant activity were fermented in PDY (Potato Dextrose Yeast) to produce large scale of the metabolites. Supernatant were extracted with ethyl acetate solvent. Ethyl acetate extract fractionated by column chromatography {SiO2, (i). chloroform –methanol = 50:1~10:1; (ii). Choloroform-Methanol = 5:1) and obtained three fractions. Further, colorimetric using free radical scavenger method was perform to assess their antioxidant activity. The highest antioxidant activity were identification by Nuclear Magnetic Resonance (1H- & 13C-NMR), infrared and mass spectroscopies showed the antioxidant compoud as flavonoid. In conclusion, the pure compound of secondary metabolites isolated from Bawang dayak leaf is flavonoid that could be responsible for its potent antioxidant activity.