Bogdan Florin Craciun
Center of Advanced Research in Bionanoconjugates and Biopolymers, Romania
Title: Self-assembled polymeric vectors for gene delivery
Biography
Biography: Bogdan Florin Craciun
Abstract
In recent decades a considerable attention was accorded to the field of drug delivery. A fraction of this domain is based on polymeric micelles with nanometer dimensions which proved their efficiency in vitro and in vivo testing. In this context, a library of self-assembled vectors based on squalene, benzene-1,3,5-tricarboxaldehyde (TA), branched polyethylenimine (PEI) and linear polyethylene glycol (PEG), was obtained as vectors for gene delivery. We have shown that this type of polymeric vectors possess a great affinity for binding the nucleic acid and good transfection efficiency on HeLa cell line. The main objective of the presented work was to find the best transfecting vector by tuning the ratios of components in the system, thus maximizing the transfection efficiency keeping a minimum grade of toxicity. We applied dynamic combinatorial chemistry for the preparation of vectors and it was proved the formation of polyplexes by gel electrophoresis and TEM. In vitro biological assessments show that the content of PEG in obtained polyplexes, plays a crucial role in transfection efficiency and cytotoxicity on HeLa cell line.